PT  - JOURNAL ARTICLE
AU  - Figueroa-Valverde, Lauro
AU  - Diaz-Cedillo, Francisco
AU  - Garcia-Cervera, Elodia
AU  - Gomez, Eduardo Pool
AU  - Lopez-Ramos, Maria
TI  - Effect of progesterone-carbachol derivative on perfusion pressure and coronary resistance in isolated rat heart: via activation of the M&lt;sub&gt;2&lt;/sub&gt; muscarinic receptor
DP  - 2014 Apr 1
TA  - Biomedical papers
PG  - 073--079
VI  - 158
IP  - 1
AID - 10.5507/bp.2012.010
IS  - 12138118
AB  - Aim: The present study was designed to investigate the effects of progesterone-carbachol derivative on perfusion pressure and coronary resistance in rats. An additional aim was to identify the molecular mechanisms involved.
Methods: The Langendorff model was used to measure perfusion pressure and coronary resistance changes in isolated rat heart after progesterone-carbachol derivative alone and after the following compounds; mifepristone (progesterone receptor blocker), yohimbine (α&lt;sub&gt;2&lt;/sub&gt; adreno-receptor antagonist), ICI 118,551 (selective β&lt;sub&gt;2&lt;/sub&gt; receptor blocker), atropine (non-selective muscarinic receptor antagonist), methoctramine (antagonist of M&lt;sub&gt;2&lt;/sub&gt; receptor) and L-NAME (inhibitor of nitric oxide synthase).
Results: The results show that progesterone-carbachol derivative [10&lt;sup&gt;-9&lt;/sup&gt; mM] significantly decreased perfusion pressure (P=0.005) and coronary resistance (P=0.006) in isolated rat heart. Additionally, the effect of progesterone-carbachol on perfusion pressure [10&lt;sup&gt;-9&lt;/sup&gt; to 10&lt;sup&gt;-4 &lt;/sup&gt;mM] was only blocked in the presence of methoctramine and L-NAME.
Conclusions: These data suggest that progesterone derivative exert its effect on perfusion pressure via activation of the M&lt;sub&gt;2&lt;/sub&gt; muscarinic. In addition, this phenomenon involves stimulation of nitric oxide synthase (NOS).