PT  - JOURNAL ARTICLE
AU  - Larocca, Nancy
AU  - Moreno, Dolores
AU  - Garmendia, Jenny Valentina
AU  - Velasquez, Olga
AU  - Martin-Rojo, Joana
AU  - Talamo, Carlos
AU  - Garcia, Alexis
AU  - De Sanctis, Juan Bautista
TI  - Beta 2 adrenergic receptor polymorphisms, at codons 16 and 27, and bronchodilator responses in adult Venezuelan asthmatic patients
DP  - 2013 Dec 12
TA  - Biomedical papers
PG  - 374--378
VI  - 157
IP  - 4
AID - 10.5507/bp.2012.084
IS  - 12138118
AB  - Background: One of the gene polymorphisms often studied in asthmatic patients is the β2 adrenergic receptor (ADRβ2). Even though in the Venezuelan Mestizo population there is a high incidence of asthma, there are no direct reports of ADRβ2 gene polymorphism, and treatment response. The aim of this study was to assess, in this population, the gene frequency of ADRβ2 polymorphisms at codons 16 Arg/Gly and 27 Gln/Glu, allergen sensitization, and its relationship to bronchodilator response.
Methods: Purified genomic DNA was obtained form 105 Mestizo asthmatic and 100 Mestizo healthy individuals from Venezuela. The two polymorphisms were assessed by PCR-RFLP. Patient sensitization to aeroallergens and their response to bronchodilatation were correlated.
Results: Significant differences between patients and controls were recorded in: 1) the prevalence of Arg/Arg at codon 16 (28.6% in patients vs. 47% in controls, P