Biomedical papers, 2016 (vol. 160), issue 1

Biomed Pap Med Fac Univ Palacky Olomouc Czech Repub. 2016, 160(1):106-110 | 10.5507/bp.2015.038

Increase of serum interleukin 6 and interferon γ is associated with the number of impulses in patients with supraventricular arrhythmias treated with radiofrequency catheter ablation

Radek Pudila, Martina Vasatovab, Petr Parizeka, Ludek Hamana, Lucie Horakovaa, Vladimir Palickab
a 1st Deptartment of Internal Medicine - Cardioangiology, Faculty of Medicine in Hradec Kralove, Charles University in Prague, Hradec Kralove, Czech Republic
b Institute of Clinical Biochemistry and Diagnostics, Faculty of Medicine in Hradec Kralove, Charles University in Prague, Hradec Kralove, Czech Republic

Background: Activation of the immune system plays a pathogenic role in the process of myocardial remodeling in patients with supraventricular arrhythmias. The intensity of this process is associated with the effectiveness of electrical cardioversion and radiofrequency catheter ablation (RFA). The aim of this study was to test the ability of the biochip microarray to detect immune parameters in patients with supraventricular arrhythmias undergoing RFA treatment.

Methods: We used a biochip-based microarray system to determine multiple immune parameters in a group of 35 patients who had undergone RFA for atrioventricular nodal reentry tachycardia (AVNRT), atrial flutter (AFL) and atrial fibrillation (AF).

Results: Before the procedure, serum IL-6 and VEGF levels were significantly increased in patients with atrial fibrillation compared to patients with AVNRT (IL-6: 6.4±6.3 ng/L vs. 1.5±0.7 ng/L, P < 0.01; VEGF: 132.4±74 ng/L vs. 88.5±56.4 ng/L, P < 0.01). After the procedure, serum IL-6, VEGF, IFN-γ and MCP-1 levels significantly increased compared to baseline (IL-6: 5.2±4.8 ng/L vs. 2.9±2.1 ng/L, P < 0.01; VEGF: 195.8±160 ng/L vs. 119.8± 110 ng/L, P < 0.05; IFN-γ: 3.1±1.2 ng/L vs. 2.3±0.6 ng/L, P < 0.05; MCP-1: 104.1±84.5 ng/L vs. 54.5±50 ng/L, P < 0.05). Serum IL-6 and IFN-γ were associated with the number of RFA applications (IL-6: r = 0.56, n 33; IFN-γ: r = 0.47, n 33).

Conclusions: This study showed that biochip-based microarray can be useful in the detection of immune activation in patients with arrhythmias and can detect myocardial injury after RF procedures.

Keywords: cytokines, cardiology, radiofrequency catheter ablation, supraventricular arrhythmia, myocardial injury

Received: November 15, 2014; Accepted: July 21, 2015; Prepublished online: September 2, 2015; Published: March 30, 2016


References

  1. Morady F. Radio-frequency ablation as treatment for cardiac arrhythmias. N Engl J Med 1999;340:534-44. Go to original source... Go to PubMed...
  2. Tanno K, Kobayashi Y, Kurano K, Kikushima S, Yazawa T, Baba T, Inoue S, Mukai H, Katagiri T. Histopathology of canine hearts subjected to catheter ablation using radiofrequency energy. Jpn Circ J 1994;58:123-35. Go to original source... Go to PubMed...
  3. Ellinor PT, Low A, Patton KK, Shea MA, Macrae CA. C-Reactive protein in lone atrial fibrillation. Am J Cardiol 2006;97:1346-50. Go to original source... Go to PubMed...
  4. Conway DS, Buggins P, Hughes E, Lip GY J. Relationship of interleukin-6 and C-reactive protein to the prothrombotic state in chronic atrial fibrillation. Am Coll Cardiol 2004;43:2075-82. Go to original source... Go to PubMed...
  5. Brueckmann M, Wolpert C, Bertsch T, Sueselbeck T, Liebetrau C, Kaden JJ, Huhle G, Neumaier M, Borggrefe M, Haase KK. Markers of myocardial damage, tissue healing, and inflammation after radiofrequency catheter ablation of atrial tachyarrhythmias. J Cardiovasc Electrophysiol 2004;15:686-91. Go to original source... Go to PubMed...
  6. Liuba I, Ahlmroth H, Jonasson L, Englund A, Jönsson A, Säfström K, Walfridsson H. Source of inflammatory markers in patients with atrial fibrillation. Europace 2008;10:848-53. Go to original source... Go to PubMed...
  7. Letsas K, Weber R, Burkle G, Mihas CC, Minners J, Kalusche D, Arentz T. Pre-ablative predictors of atrial fibrillation recurrence following pulmonary vein isolation: the potential role of inflammation. Europace 2009;11:158-63. Go to original source... Go to PubMed...
  8. Issac T, Dokaninish H, Lakkish MM. Role of inflammation in initiation and perpetuation of atrial fibrillation: a systematic review of the published data. J Am Coll Cardiol 2007;50:2021-8. Go to original source... Go to PubMed...
  9. Stein A, Wessling G, Diesenhofer I, Busch G, Steppich B, Estner H, Zrenner B, Schmitt C, Braun S, Schomig A, Ott I. Systemic inflammatory changes after pulmonary vein radiofrequency ablation do not alter stem cell mobilization. Europace 2008;10:444-9. Go to original source... Go to PubMed...
  10. Marcus GM, Smith L, Glidden D, Wilson E, McCabe JM, Whiteman D, Tseng ZH, Badhwar N, Lee BK, Lee RJ, Scheinman MM, Olgin JE. Markers of Inflammation Before and After Curative Ablation of Atrial Flutter. Heart Rhythm 2008;5:215-21. Go to original source... Go to PubMed...
  11. Giannitsis E, Kurz K, Hallermayer K, Jarausch J, Jaffe A, Katus HA. Analytical validation of a high-sensitivity cardiac troponin T assay. Clin Chem 2010;56:254-61. Go to original source... Go to PubMed...
  12. Frustaci A, Chimenti C, Bellocci F, Morgante E, Russo AM, Maseri A. Histological substrate of atrial biopsies in patients with lone atrial fibrillation. Circulation 1997; 96:1180-4. Go to original source... Go to PubMed...
  13. Aviles RJ, Martin DO, Apperson-Hansen C, Houghtaling PL, Rautaharju P, Kronmal RA, Tracy RP, Van Wagoner DR, Psaty BM, Lauer MS, Chung MK. Inflammation as a Risk Factor for Atrial Fibrillation. Circulation 2003;104:2886. Go to original source... Go to PubMed...
  14. Emkanjoo Z, Mottayden M, Givtaj N, Alasti M, Arya A, Haghjoo M, Fazelifar AF, Alizadeh A, Sadr-Ameli MA. Evaluation of post-radiofrequency myocardial injury by measuring cardiac troponin I levels. Int J Cardiol 2007;117:173-7. Go to original source... Go to PubMed...
  15. Vasatova M, Pudil R, Tichy M, Buchler T, Horacek JM, Haman L, Parizek P, Palicka V. High-sensitivity troponin T as a marker of myocardial injury after radiofrequency catheter ablation. Ann Clin Biochem 2011;48:38-40. Go to original source... Go to PubMed...
  16. Haines DE, Whayne JG, Walker J, Nath S, Bruns D. The effect of radiofrequency catheter ablation on myocardial creatine kinase activity. J Cardiovasc Electrophysiol 1995;6:79-88. Go to original source... Go to PubMed...
  17. Pudil R, Pařízek P, Tichy M, Haman L, Horaková L, Ulrychova M, Vojacek J, Palicka V. Use of the biochip microarray system in detection of myocardial injury caused by radiofrequency catheter ablation. Clin Chem Lab Med 2008;46:1726-8. Go to original source... Go to PubMed...