Biomedical papers, 2015 (vol. 159), issue 1

Biomed Pap Med Fac Univ Palacky Olomouc Czech Repub. 2015, 159(1):060-066 | 10.5507/bp.2014.052

Selected 68Ga-siderophores versus 68Ga-colloid and 68Ga-citrate: biodistribution and small animal imaging in mice

Milos Petrika, Adela Vlckovaa, Zbynek Novya, Lubor Urbanekb, Hubertus Haasc, Clemens Decristoforod
a Institute of Molecular and Translational Medicine, Faculty of Medicine and Dentistry, Palacky University Olomouc, Czech Republic
b Laboratory of Growth Regulators, Centre of the Region Hana for Biotechnological and Agricultural Research, Institute of Experimental Botany AS CR and Palacky University Olomouc
c Division of Molecular Biology/Biocenter, Innsbruck Medical University, Innsbruck, Austria
d Clinical Department of Nuclear Medicine, Innsbruck Medical University, Innsbruck, Austria

Background: 68Ga-triacetylfusarinine C (TAFC) and 68Ga-ferrioxamine E (FOXE) show great potential to be used as highly sensitive and selective tracers for Aspergillus infection imaging. Here we report on a comparison of the ex vivo biodistribution and small animal imaging of 68Ga-TAFC and 68Ga-FOXE versus 68Ga-colloid and 68Ga-citrate as unspecific control in mice.

Methods: The radiochemical purity of tested 68Ga labelled tracers was determined by RP-HPLC or ITLC-SG. Ex vivo biodistribution was studied in normal DBA/2 mice 30 min and 90 min p.i. Static and dynamic imaging were performed using µPET/CT.

Results: 68Ga-TAFC and 68Ga-FOXE showed rapid renal excretion and low blood values even 90 min p.i. 68Ga-TAFC showed almost no retention in other organs while 68Ga-FOXE displayed some uptake in gastrointestinal tract. 68Ga-colloid and 68Ga-citrate revealed significantly different ex vivo biodistribution. 68Ga-colloid showed pronounced radioactivity retention in the liver, while 68Ga-citrate displayed high blood values and significant retention of radioactivity in highly perfused organs.

Conclusions: From the results, both 68Ga-TAFC and 68Ga-FOXE have excellent and significantly different in vivo behaviour compared to 68Ga-colloid and 68Ga-citrate. 68Ga-TAFC in particular confirmed its great potential use as a specific tracer for Aspergillus infection imaging.

Keywords: gallium-68, siderophores, colloid, citrate, µPET imaging, aspergillosis

Received: August 11, 2014; Accepted: September 30, 2014; Prepublished online: October 29, 2014; Published: March 9, 2015


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