Biomedical papers, 2013 (vol. 157), issue 4

Biomed Pap Med Fac Univ Palacky Olomouc Czech Repub. 2013, 157(4):284-293 | 10.5507/bp.2013.057

Expression of miR-15a and miR-16-1 in patients with chronic lymphocytic leukemia

Lenka Humplikovaa, Sona Kollinerovab, Tomas Papajika, Zuzana Pikalovaa, Milena Holzerovaa, Vit Prochazkaa, Martina Divokaa, Martin Modrianskyb, Karel Indraka, Marie Jarosovaa
a Department of Hemato-Oncology, Faculty of Medicine and Dentistry, Palacky University Olomouc, Czech Republic
b Department of Medical Chemistry and Biochemistry, Faculty of Medicine and Dentistry, Palacky University Olomouc

Introduction: MicroRNAs (miRNAs) are small non-coding single-stranded RNA molecules that regulate gene expression at the post-transcriptional level. In the pathogenesis of chronic lymphocytic leukemia (CLL), miR-15a and miR-16-1 play an important role. These miRNAs are located on chromosome 13 in the 13q14.3 region, which is deleted in more than 55% of CLL patients. This aberration affects expression of miRNAs.

Objectives: The study aimed at performing a molecular genetic analysis of miR-15a and miR-16-1 expression in a group of 39 patients diagnosed with CLL and determining the association between the expression of the two miRNAs and types of deletions in the 13q14 region.

Methods: We used fluorescence in situ hybridiziation (FISH) for determination of mono- or biallelic deletion 13q and quantitative polymerase chain reaction (Q-RT-PCR) to revealed expression miR-15a and miR-16-1 in 39 patients suffering from CLL.

Results: The analysis comprised 19 patients with monoallelic 13q14 deletion, 3 patients with biallelic deletion, 9 patients with both monoallelic and biallelic deletions, and 8 patients without 13q14 deletion serving as controls. The results showed different levels of miRNA expression in individual patients. Significantly higher normalized levels of miR-15a expression were found in the control group and patients with monoallelic 13q14 expression compared with patients with biallelic deletion. There was a significantly decreased expression of both miRNAs in patients with biallelic deletion of the 13q14 region but only when deletions were present in 77% or more of cells, as detected by fluorescent in situ hybridization (FISH).

Keywords: chronic lymphocytic leukemia, microRNA, miRNA expression, fluorescence in situ hybridization,13q14 deletion, monoallelic deletion, biallelic deletion

Received: January 8, 2013; Accepted: July 19, 2013; Prepublished online: September 5, 2013; Published: December 12, 2013


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