Biomedical Papers, 2013 (vol. 157), issue 2

Biomed Pap Med Fac Univ Palacky Olomouc Czech Repub. 2013, 157(2):137-145 | 10.5507/bp.2013.036

Differential modulation of inflammatory markers in plasma and skin after single exposures to UVA or UVB radiation in vivo

Jitka Vostalovaa, Alena Rajnochova Svobodovaa, Adela Galandakovaa, Jarmila Sianskaa, Dalibor Dolezalb, Jitka Ulrichovaa
a Department of Medical Chemistry and Biochemistry, Faculty of Medicine and Dentistry, Palacky University Olomouc, Czech Republic
b Center for Laboratory Animals, Faculty of Medicine and Dentistry, Palacky University Olomouc

Background: Solar light generates inflammatory responses in exposed skin. These effects are generally attributed to UVB light. However, skin is expose d to a huge quantum of UVA photons as UVA is a predominant part of sunlight and the radiation used in tanning beds. We examined the effects of a single exposure to UVA and UVB wavebands on cytokine levels in skin and plasma, myeloperoxidase (MPO) activity, expression of inducible nitric oxide synthase (iNOS) and cyclooxygenase 2 (COX-2) in skin.

Methods: Hairless mice were irradiated with either UVA (10 or 20 J/cm2) or UVB (200 or 800 mJ/cm2). The effects were assessed after 4/24 h. Plasma cytokine levels were evaluated using a Bio-Plex cytokine assay. Cytokine, iNOS and COX-2 levels in skin were determined by Western blot. Skin MPO activity was monitored spectrophotometrically.

Results: UVB induced up-regulation of interleukin-1β (IL-1β) and interleukin-6 (IL-6) and decrease in interleukin-10 (IL-10) mainly after 4 h. In contrast, UVA caused increase in levels of tumor necrosis factor-alpha (TNF-α) and IL-6 after 4 h and up-regulated IL-10 and interleukin-12 (IL-12) after 24 h. The increase in MPO activity from infiltrated leucocytes was observed only in UVB irradiated animals. iNOS was up-regulated 4 h after UVA and UVB treatment. No significant effect on COX-2 expression was detected.

Conclusions: UVA and UVB light affected several inflammatory markers. For individual waveband, changes in plasma parameters did not correlate with those in skin. Thus evaluation of plasma samples cannot simply be replaced by determination in skin specimens and vice versa.

Keywords: solar radiation, hairless mice, cytokine, myeloperoxidase, nitric oxide synthase, cyclooxygenase-2

Received: November 20, 2012; Accepted: April 30, 2013; Published: June 3, 2013


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