Biomedical papers, 2006 (vol. 150), issue 1

Biomed Pap Med Fac Univ Palacky Olomouc Czech Repub. 2006, 150(1):51-61 | 10.5507/bp.2006.005

MOLECULAR-GENETIC APPROACHES TO IDENTIFICATION AND TYPING OF PATHOGENIC CANDIDA YEASTS

Jitka Trtkovaa, Vladislav Raclavskyb
a Department of Biology, Faculty of Medicine, Palacký University, Hněvotínská 3, 775 15 Olomouc, Czech Republic
b Department of Microbiology, Faculty of Medicine, Palacký University, Hněvotínská 3, 775 15 Olomouc, Czech Republic

Currently, invasive candidal infections represent an increasing cause of morbidity and mortality in seriously ill hospitalised patients. Because the accurate diagnosis of candidiasis remains difficult, a fast and reliable assay for characterization of fungal pathogens is critical for the early initiation of adequate antifungal therapy and/or for introduction of preventive measures. As novel molecular genetic techniques are continuously introduced, their role in the management of infectious diseases has also been growing. Today, molecular strategies complement conventional methods and provide more accurate and detailed insight. It can be expected that future technical development will improve their potential furthermore. In this article, we provide a critical review on the value and limitations of molecular tools in pathogenic Candida species identification and strain typing regarding their sensitivity, discriminatory power, reproducibility, cost and ease of performance.

Keywords: Candida, Infection, Typing, Molecular methods, PCR, Melting analysis

Received: April 21, 2006; Accepted: June 5, 2006; Published: July 1, 2006


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